To let researcher check the true identity of their cell lines a requirement increasingly imposed by scientific journals and funding agencies the dsmz offers a cell line authentication service. Database of crosscontaminated or misidentified cell lines compiled by the international cell line authentication committee iclac. Use of a consensus str panel now enables multicenter interactive searches, work piloted at the dsmz. Authentication of human cell lines leibnizinstitut dsmz. Cal851 cell line hms lincs database hms lincs project. Str profiling is shown to be ideal for authentication of human cell line samples. These databases contain human cell line str profiles and are essential. Cell line panels were established for basic research and drug development, but. Welcome to the leibniz institute dsmz german collection of microorganisms and cell cultures gmbh. Which techniques do you use for cell lines authentication.
Guidelines for the use of cell lines in biomedical. A total of 436 available human cell lines were obtained by the dsmz braunschweig. Record all data relevant to the origin of the tissue when starting a new cell line and keep tissue for dna profiling. To validate an order a signed agreement form has to be sent to us via fax. Cell line authentication, provenance and availability are what you are after, but of course easier said than done. The dsmz cell lines collection currently comprises more than 840 different immortalized cell cultures of primate, rodent, amphibian, fish, and insect origin isolated from numerous tissues as well as a number of hybridomas. Which techniques do you use for cell lines authentication and why. An interactive reference database of str profiles covering common cancer cell lines article in international journal of cancer 1261. Cell lines are usually populations of cells that are actively evolving with time in. The dsmz, together with the atcc, jcrb, and riken repositories, have generated comprehensive databases of short tandem repeats str cell line pro. German collection of microorganisms and cell cultures gmbh.
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